Translational_Unit

Part:BBa_K4643017:Design

Designed by: Baptiste Rivoirard   Group: iGEM23_Sorbonne-U-Paris   (2023-10-10)


CYP52M1 Brick


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal BglII site found at 308
    Illegal BamHI site found at 1278
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 66


Design Notes

No design considerations


Source

It comes from the genomic sequence, for more information see the CYP52M1 part

References